lymphocyte model  (ATCC)

Journal: Current Issues in Molecular Biology

Article Title: Development of a Novel Immunoprotective Culture System for Parathyroid Allografts: Utilizing Static Magnetic Fields to Modulate Lymphocyte Migration

doi: 10.3390/cimb48040388

Figure Lengend Snippet: Qualitative Transcriptional Profiling of Jurkat T-Lymphocyte-Specific Biomarkers via Semi-Quantitative RT-PCR. ( A – C ) Electrophoretic analysis of IL-1α, IL-1β, and IL-2 genes in Jurkat cell populations at 24 and 72 h for ( A ) groups 5–6 (lymphocytes only), ( B ) groups 7–8 (non-encapsulated co-culture), and ( C ) groups 9–10 (encapsulated co-culture). Molecular sizes are expressed in base pairs (bp), representing the specific PCR amplicon lengths for each primer set. All visible bands matched the predicted nucleotide lengths for IL-1α (147 bp) and β-actin (142 bp), confirming target-specific gene amplification across all Jurkat-containing groups.

Article Snippet: The Jurkat cell line (ATCC, #TIB-152TM) served as the lymphocyte model. Jurkat cells were cultured in complete RPMI-1640 (1X, Gibco, Waltham, MA, USA) medium supplemented with 10% ( v / v ) FBS and 1% ( v / v ) P/S.

Techniques: Quantitative RT-PCR, Co-Culture Assay, Amplification

Journal: Current Issues in Molecular Biology

Article Title: Development of a Novel Immunoprotective Culture System for Parathyroid Allografts: Utilizing Static Magnetic Fields to Modulate Lymphocyte Migration

doi: 10.3390/cimb48040388

Figure Lengend Snippet: Spatiotemporal Analysis of Jurkat Cell Migration Dynamics via Live-Cell Imaging. ( A , B ) Representative bright-field micrographs of Group 9 (encapsulated parathyroid cells co-cultured with Jurkat lymphocytes in the absence of SMF) at 24 ( A , C ) and 72 ( B , D ) h, illustrating a randomized lymphocyte distribution at the capsule interface. ( C , D ) Corresponding images of Group 10 (SMF-exposed) demonstrating directional magnetophoretic migration and the emergence of distinct lymphocyte-depleted zones surrounding the alginate capsule. Magnification: 2.5×. Black arrows denote the sodium alginate microcapsule boundary; black arrowheads indicate Jurkat cell populations. Note: Dynamic mobilization and altered spatial distribution vectors are further documented in .

Article Snippet: The Jurkat cell line (ATCC, #TIB-152TM) served as the lymphocyte model. Jurkat cells were cultured in complete RPMI-1640 (1X, Gibco, Waltham, MA, USA) medium supplemented with 10% ( v / v ) FBS and 1% ( v / v ) P/S.

Techniques: Migration, Live Cell Imaging, Cell Culture

Journal: Current Issues in Molecular Biology

Article Title: Development of a Novel Immunoprotective Culture System for Parathyroid Allografts: Utilizing Static Magnetic Fields to Modulate Lymphocyte Migration

doi: 10.3390/cimb48040388

Figure Lengend Snippet: Comparative Evaluation of Lymphocyte Positioning in Non-Encapsulated Co-Culture Systems. ( A , B ) Micrographs of Group 7 (non-encapsulated parathyroid cells + Jurkat cells; SMF-) and ( C , D ) Group 8 (non-encapsulated parathyroid cells + Jurkat cells; SMF+) after 24 ( A , C ) and 72 ( B , D ) hours of incubation. In the absence of an alginate physical barrier, no significant directional migration or separation was observed regardless of magnetic field application, highlighting the essential synergistic role of the combinatorial encapsulation–SMF approach for immune cell modulation. Magnification: 10×. Arrows indicate non-encapsulated parathyroid cell clusters; arrowheads denote Jurkat cell distribution.

Article Snippet: The Jurkat cell line (ATCC, #TIB-152TM) served as the lymphocyte model. Jurkat cells were cultured in complete RPMI-1640 (1X, Gibco, Waltham, MA, USA) medium supplemented with 10% ( v / v ) FBS and 1% ( v / v ) P/S.

Techniques: Co-Culture Assay, Incubation, Migration, Encapsulation

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: In silico model of adrenergic [1A] and estrogen [1B] signalling cascades in lymphocytes: The cross-talk between the adrenergic signals (1A) and estrogen-mediated signals (1B) result in specific immunomodulatory effects depending upon the estrogen concentration. The layout of the signalling pathway is elucidated from receptors to transduction into the cytosol and nuclear translocation of signalling molecules leading to specific outcomes (1C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: In Silico, Concentration Assay, Transduction, Translocation Assay

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of molecular markers p-ERK, p-CREB, cAMP and p-Akt by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (2A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (2B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (2C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of cytokines (IL-2 and IFN-g) by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (3A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (3B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (3C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of antioxidant enzymes (CAT and SOD), superoxides and peroxynitrites by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (4A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (4B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (4C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of survival/apoptosis signals by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (5A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (5B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (5C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Effects of increasing doses of estrogen on survival/apoptosis signals by terbutaline, phenylephrine and clonidine: Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of increasing doses of estrogen (10-6 M (6A), 10-5 M (6B), 10-4 M (6C), 10-3 M (6D)) for 72 hours.

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: